"aufgewärmt" - mögliche Ursachen M.Crohn

[Uli]

Altes Wissen – bisher? negiert! Oder „ belächelt“ – oder gar bekämpft!

http://www.lebensmittelallergie.info/ind...pic=1721.0

J Appl Microbiol. 2011 Feb;110(2):479-89. doi: 10.1111/j.1365-2672.2010.04905.x. Epub 2010 Dec 13.
Detection of Mycobacterium avium ssp. paratuberculosis in cheese, milk powder and milk using IS900 and f57-based qPCR assays.

Donaghy JA, Johnston J, Rowe MT.

Agri-Food and Biosciences Institute (AFBI), AFESD, Newforge Lane, Belfast, UK. john.donaghy@afbini.gov.uk
Abstract

AIMS: To develop a quantitative PCR assay for sensitive and specific detection of Mycobacterium avium ssp. paratuberculosis (Map) in a range of dairy products.

METHODS AND RESULTS: TaqMan() assays were designed to target the IS900 and f57 genetic elements of Map. Both real-time PCR assays were integrated with the Adiapure() Map DNA extraction kit and assessed separately for the detection/quantification of Map in spiked milk, Cheddar cheese and milk powder. Assays were validated against Cheddar cheese samples containing known concentrations of Map. The IS900 qPCR assay was significantly more sensitive than the assay based on the f57 primer/probe. At a threshold cycle value of 38, limits of detection (LOD) for the IS900 qPCR assay were 0·6 CFU ml(-1), 2·8 CFU g(-1) and 30 CFU g(-1) for artificially contaminated pasteurized milk, whole milk powder and Cheddar cheese, respectively. The respective LOD's for the f57 assay were 6·2 CFU ml(-1), 26·7 CFU g(-1) and 316 CFU g(-1).

CONCLUSION: The integrated Adiapure() extraction - IS900 real time assay described is a sensitive, quantitative method for the detection of Map in dairy products. This is the first study to consider qPCR as a quantitative estimation of Map-DNA in cheese and whole milk powder.

SIGNIFICANCE AND IMPACT OF THE STUDY: The assay developed allows sensitive detection and quantification of Map DNA in a range of dairy products which is valuable for the screening and surveillance of this potential zoonotic organism.
2010 The Authors. Journal of Applied Microbiology 2010 The Society for Applied Microbiology.
http://www.ncbi.nlm.nih.gov/pubmed?term=%2021155954

….bisher war es sehr schwierig, MAP? nachzuweisen – sie haben sich den bisherigen Labosuntersuchungen “ entzogen”…….

BMC Res Notes. 2010 Dec 19;3:339.
Genetic adult lactase persistence is associated with risk of Crohn's Disease in a New Zealand population.

Nolan DJ, Han DY, Lam WJ, Morgan AR, Fraser AG, Tapsell LC, Ferguson LR.

Smart Foods Centre, University of Wollongong, Northfields Ave, Wollongong, New South Wales, Australia. djn297@uow.edu.au.
Abstract


BACKGROUND: Mycobacterium avium subspecies paratuberculosis (MAP) is an infective agent found in ruminants and milk products, which has been suggested to increase the risk of gastrointestinal inflammation in genetically susceptible hosts. It is hypothesized that lactase persistence facilitates exposure to such milk products increasing the likelihood of adverse outcomes. Individuals either homozygous or heterozygous for the T allele of DNA variant, rs4988235, located 14kb upstream from the LCT locus, are associated with having lactase persistence.The aim of this study was to determine whether lactase persistence as evident by the T allele of rs4988235 is associated with Crohn's Disease (CD) in a New Zealand population.

FINDINGS: Individuals homozygous for the T allele (T/T genotype) showed a significantly increased risk of having CD as compared with those homozygous for the C allele (OR = 1.61, 95% CI = 1.03-2.51). Additionally, a significant increase in the frequency of the T allele was observed in CD patients (OR = 1.30, 95% CI = 1.05-1.61, p = 0.013), indicating that the T allele encoding lactase persistence was associated with an increased risk of CD.

CONCLUSIONS: Our findings indicate that lactase persistence as evident by the presence of the T allele of rs4988235 is associated with risk of CD in this New Zealand Caucasian population.
http://www.ncbi.nlm.nih.gov/pubmed?term=%2021167073%20

mhhh – hier zeigt sich wohl, dass die “Laktose-TOLERANTEN”? - also die Milchkonsumenten – wohl eher zu CED neigen als die Laktose-INTOLERANTEN, die auf Milch(-produkte) verzichten! Und ich denke, dass das nicht nur in Neuseeland so sein wird........

Aber : es wird ja auch vermehrt um Milch geworben........
Siehe hier:
http://www.proplanta.de/Agrar-Nachrichte...07656.html

Ohhh nein – wir LI`ler? müssen / sollen nicht auf Milch(-produkte) verzichten.........


Und : die „ zauberhafte Milch“
http://www.proplanta.de/Agrar-Nachrichte...31875.html



Quintessenz dieses Beitrags sollte sein: Sich als Betroffener genau informieren , sich nicht nur auf Aussagen von Ärzten und Ernährungsberater verlassen! Auf „ den Bauch hören“ ....nicht auf Broschüren & Co! Evtl. auch Allergietests machen lassen......

Uli