17.12.2004, 13:14
Rindfleischallergie
Allergy. 2000 Apr;55(4):348-54.
Identification of bovine IgG as a major cross-reactive vertebrate meat allergen.
Ayuso R, Lehrer SB, Lopez M, Reese G, Ibanez MD, Esteban MM, Ownby DR, Schwartz H.
Section of Allergy and Clinical Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
BACKGROUND: Although beef is a main source of protein in Western diets, very little has been published on allergic reactions to beef or the main allergens implicated in these reactions. The aim was to evaluate the IgE antibody response to beef in suspected meat-allergic subjects and assess cross-reactivity of beef with other vertebrate meats. METHODS: Fifty-seven sera from suspected meat-allergic subjects were tested by grid blot for specific IgE antibodies to vertebrate meats (beef, lamb, pork, venison, and chicken), and the patterns of recognition of meat proteins were assessed by immunoblot studies. RESULTS: A 160-kDa band, identified as bovine IgG , was detected in raw beef in 83% (10/12) of beef-allergic subjects but in only 24% of the beef-tolerant subjects. IgE reactivity to a band of similar mol. mass was detected also in lamb and venison, but rarely in pork or chicken. Complete inhibition of the IgE reactivity to the bovine IgG was obtained with lamb, venison, and milk. IgE reactivity to this band also completely disappeared when beef or lamb extracts were separated under reducing conditions, indicating conformational epitopes. CONCLUSIONS: Bovine IgG appears to be a major cross-reacting meat allergen that could predict beef allergy . Further studies with oral IgG challenges should be performed to document the conclusion that in vitro reactivity correlates with clinical hypersensitivity. The role of bovine IgG in other bovine products such as milk, dander, or hair must also be studied, and the hypothesis that it is a cross-reacting allergen with other mammalian products validated.
PMID: 10782519 [PubMed - indexed for MEDLINE]
( merkwürdig: hier werden IgG-Reaktionen anerkannt – und “unsre Verantwortlichen” versuchen mit aller Gewalt , IgG schlecht zu machen, oder als "Geldschneiderei" anzuprangern ......)
******************
1: Nutrition. 2000 Jun;16(6):454-7.
Beef allergy in children.
Fiocchi A, Restani P, Riva E.
Department of Pediatrics, San Paolo Biomedical Institute, University of Milan Medical School, Milan, Italy.
Beef allergy was poorly known before the '90s. Since then, a number of papers appeared elucidating the nature, epidemiology, and symptoms of beef allergy in children allergic to cow's milk and children suffering from atopic dermatitis. It is now clear that beef allergy is not an infrequent occurrence, with an incidence between 3. 28% and 6.52% among children with atopic dermatitis, its incidence may be as much as 0.3% in the general population. A diagnosis of beef allergy must be supported by skin prick tests, RASTs, and challenges. The specificity and sensitivity according to type of test and the type of extract, however, remains to be evaluated. Despite the fact that other allergens can be sensitizing, the major beef allergen is bovine serum albumin (BSA). Beef-sensitive children are also sensitized to ovine serum albumin, as well as to other serum albumins; therefore, the use of alternative meats in beef-allergic children must be carefully evaluated on an individual basis. Because industrial heat processing is more efficient than domestic cooking in reducing reactivity in beef-sensitive children, freeze-drying and homogenization may support the introduction of processed beef into the diet of beef-allergic children.
Publication Types:
· Review
· Review, Tutorial
PMID: 10869903 [PubMed - indexed for MEDLINE]
Hier könnte wohl mit ein Grund liegen, dass man mit einer Allergie auf bovines Serumalbumin ( = Molkenprotein) auch Allergien und IgG-vermittelte Unverträglichkeiten auf andere Fleischsorten und auch Hühnereiweiß entwickeln kann.
Und ich weiß nicht, ob das verwegen von mir ist zu behaupten: mit der Milch beginnt`s! Ich denke nämlich, dass Säuglinge und/oder Kleinkinder seltenst Rindersteaks in ihrem Speiseplan haben!
*****************
1: Allergy. 2004 Aug;59 Suppl 78:21-4.
Characterization of bovine serum albumin epitopes and their role in allergic reactions.
Restani P, Ballabio C, Cattaneo A, Isoardi P, Terracciano L, Fiocchi A.
Department of Pharmacological Sciences, University of Milan, Italy.
OBJECTIVE: This review provides updated information on conformational and sequential epitopes identified in bovine serum albumin (BSA) and summarizes available data about the role of structural modifications on BSA antigenicity/allergenicity. DATA SOURCES: Data on beef allergy and BSA antigenicity are reported, with reference both to the basic literature and to clinical results obtained by our group. RESULTS AND DISCUSSION: BSA ( bovines Serum Albumin) is an important allergen involved in milk and beef allergy . The presence of conformational epitopes has been suggested by indirect evidence, while at least one sequential epitope has been experimentally identified. The role of structural modifications on BSA antigenicity is discussed as well as the increased tolerance observed in allergic subjects consuming beef as strained (homogenized) and freeze-dried derivatives. CONCLUSION: Study of the molecular characteristics of a known major allergen allows the identification of technological processes that may be capable of improving the tolerance of allergic subjects to a specific food. Even though any hoped for reduced allergenicity must be verified under medical supervision, the use of new products could obviate the need to avoid important foods such as meat in childhood. Copyright 2004 Blackwell Munksgaard
Publication Types:
· Review
· Review, Tutorial
PMID: 15245352 [PubMed - indexed for MEDLINE]
****************
Und noch zuletzt eine Publikation aus Japan : hier wird aber merkwürdigerweise der Zusammenhang zur Kuhmilchallergie “verschwiegen” –vielleicht setzen sie das Wissen um diesen Zusammenhang voraus????
Allerdings wird betont, dass T-Zellen für Reaktionen wohl mitverantwortlich zu machen sind!
Biochem Biophys Res Commun. 2002 May 24;293(5):1348-53.
Some human B and T cell epitopes of bovine serum albumin, the major beef allergen.
Tanabe S, Kobayashi Y, Takahata Y, Morimatsu F, Shibata R, Nishimura T.
Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan. stanabe@hiroshima-u.ac.jp
Bovine serum albumin (BSA) is the major beef allergen. Since IgE and T cell recognitions are central to the specific immune response to allergens, the identification and immunologic characterization of B and T cell epitopes of BSA represent important steps in the development of treatments for beef allergy. Prior to our experiments, we hypothesized that BSA-specific antibodies and T cells react primarily with sequential epitopes in which the amino acid sequences differ greatly between bovine and human albumin. To clarify this hypothesis, 16 peptides corresponding to such regions were synthesized as candidate epitopes. Among them, at least two regions, aa336-345 and aa451-459, were found to be B cell (IgE-binding) epitopes. In inhibition ELISA experiments, EYAV (aa338-341) and LILNR (aa453-457) bound to patient IgE antibodies and were found to be the cores of the IgE-binding epitopes. Three regions, DDSPDLPKLKPDPNTLC (aa107-123), PHACYTSVFDKLKHLVDEP (aa364-382), and LSLILNRLC (aa451-459), were found to induce T cell proliferation in more than half of the patients tested. Of interest was that these three regions were also recognized by B cells. Information concerning human B and T cells epitopes can contribute greatly to the elucidation of the etiology of beef allergy.
PMID: 12054661 [PubMed - indexed for MEDLINE]
******************
Ob es nicht bezeichnend ist, dass es keine Publikation dazu in Deutscher Sprache gibt???????
fragt sich
Uli
Allergy. 2000 Apr;55(4):348-54.
Identification of bovine IgG as a major cross-reactive vertebrate meat allergen.
Ayuso R, Lehrer SB, Lopez M, Reese G, Ibanez MD, Esteban MM, Ownby DR, Schwartz H.
Section of Allergy and Clinical Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
BACKGROUND: Although beef is a main source of protein in Western diets, very little has been published on allergic reactions to beef or the main allergens implicated in these reactions. The aim was to evaluate the IgE antibody response to beef in suspected meat-allergic subjects and assess cross-reactivity of beef with other vertebrate meats. METHODS: Fifty-seven sera from suspected meat-allergic subjects were tested by grid blot for specific IgE antibodies to vertebrate meats (beef, lamb, pork, venison, and chicken), and the patterns of recognition of meat proteins were assessed by immunoblot studies. RESULTS: A 160-kDa band, identified as bovine IgG , was detected in raw beef in 83% (10/12) of beef-allergic subjects but in only 24% of the beef-tolerant subjects. IgE reactivity to a band of similar mol. mass was detected also in lamb and venison, but rarely in pork or chicken. Complete inhibition of the IgE reactivity to the bovine IgG was obtained with lamb, venison, and milk. IgE reactivity to this band also completely disappeared when beef or lamb extracts were separated under reducing conditions, indicating conformational epitopes. CONCLUSIONS: Bovine IgG appears to be a major cross-reacting meat allergen that could predict beef allergy . Further studies with oral IgG challenges should be performed to document the conclusion that in vitro reactivity correlates with clinical hypersensitivity. The role of bovine IgG in other bovine products such as milk, dander, or hair must also be studied, and the hypothesis that it is a cross-reacting allergen with other mammalian products validated.
PMID: 10782519 [PubMed - indexed for MEDLINE]
( merkwürdig: hier werden IgG-Reaktionen anerkannt – und “unsre Verantwortlichen” versuchen mit aller Gewalt , IgG schlecht zu machen, oder als "Geldschneiderei" anzuprangern ......)
******************
1: Nutrition. 2000 Jun;16(6):454-7.
Beef allergy in children.
Fiocchi A, Restani P, Riva E.
Department of Pediatrics, San Paolo Biomedical Institute, University of Milan Medical School, Milan, Italy.
Beef allergy was poorly known before the '90s. Since then, a number of papers appeared elucidating the nature, epidemiology, and symptoms of beef allergy in children allergic to cow's milk and children suffering from atopic dermatitis. It is now clear that beef allergy is not an infrequent occurrence, with an incidence between 3. 28% and 6.52% among children with atopic dermatitis, its incidence may be as much as 0.3% in the general population. A diagnosis of beef allergy must be supported by skin prick tests, RASTs, and challenges. The specificity and sensitivity according to type of test and the type of extract, however, remains to be evaluated. Despite the fact that other allergens can be sensitizing, the major beef allergen is bovine serum albumin (BSA). Beef-sensitive children are also sensitized to ovine serum albumin, as well as to other serum albumins; therefore, the use of alternative meats in beef-allergic children must be carefully evaluated on an individual basis. Because industrial heat processing is more efficient than domestic cooking in reducing reactivity in beef-sensitive children, freeze-drying and homogenization may support the introduction of processed beef into the diet of beef-allergic children.
Publication Types:
· Review
· Review, Tutorial
PMID: 10869903 [PubMed - indexed for MEDLINE]
Hier könnte wohl mit ein Grund liegen, dass man mit einer Allergie auf bovines Serumalbumin ( = Molkenprotein) auch Allergien und IgG-vermittelte Unverträglichkeiten auf andere Fleischsorten und auch Hühnereiweiß entwickeln kann.
Und ich weiß nicht, ob das verwegen von mir ist zu behaupten: mit der Milch beginnt`s! Ich denke nämlich, dass Säuglinge und/oder Kleinkinder seltenst Rindersteaks in ihrem Speiseplan haben!
*****************
1: Allergy. 2004 Aug;59 Suppl 78:21-4.
Characterization of bovine serum albumin epitopes and their role in allergic reactions.
Restani P, Ballabio C, Cattaneo A, Isoardi P, Terracciano L, Fiocchi A.
Department of Pharmacological Sciences, University of Milan, Italy.
OBJECTIVE: This review provides updated information on conformational and sequential epitopes identified in bovine serum albumin (BSA) and summarizes available data about the role of structural modifications on BSA antigenicity/allergenicity. DATA SOURCES: Data on beef allergy and BSA antigenicity are reported, with reference both to the basic literature and to clinical results obtained by our group. RESULTS AND DISCUSSION: BSA ( bovines Serum Albumin) is an important allergen involved in milk and beef allergy . The presence of conformational epitopes has been suggested by indirect evidence, while at least one sequential epitope has been experimentally identified. The role of structural modifications on BSA antigenicity is discussed as well as the increased tolerance observed in allergic subjects consuming beef as strained (homogenized) and freeze-dried derivatives. CONCLUSION: Study of the molecular characteristics of a known major allergen allows the identification of technological processes that may be capable of improving the tolerance of allergic subjects to a specific food. Even though any hoped for reduced allergenicity must be verified under medical supervision, the use of new products could obviate the need to avoid important foods such as meat in childhood. Copyright 2004 Blackwell Munksgaard
Publication Types:
· Review
· Review, Tutorial
PMID: 15245352 [PubMed - indexed for MEDLINE]
****************
Und noch zuletzt eine Publikation aus Japan : hier wird aber merkwürdigerweise der Zusammenhang zur Kuhmilchallergie “verschwiegen” –vielleicht setzen sie das Wissen um diesen Zusammenhang voraus????
Allerdings wird betont, dass T-Zellen für Reaktionen wohl mitverantwortlich zu machen sind!
Biochem Biophys Res Commun. 2002 May 24;293(5):1348-53.
Some human B and T cell epitopes of bovine serum albumin, the major beef allergen.
Tanabe S, Kobayashi Y, Takahata Y, Morimatsu F, Shibata R, Nishimura T.
Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan. stanabe@hiroshima-u.ac.jp
Bovine serum albumin (BSA) is the major beef allergen. Since IgE and T cell recognitions are central to the specific immune response to allergens, the identification and immunologic characterization of B and T cell epitopes of BSA represent important steps in the development of treatments for beef allergy. Prior to our experiments, we hypothesized that BSA-specific antibodies and T cells react primarily with sequential epitopes in which the amino acid sequences differ greatly between bovine and human albumin. To clarify this hypothesis, 16 peptides corresponding to such regions were synthesized as candidate epitopes. Among them, at least two regions, aa336-345 and aa451-459, were found to be B cell (IgE-binding) epitopes. In inhibition ELISA experiments, EYAV (aa338-341) and LILNR (aa453-457) bound to patient IgE antibodies and were found to be the cores of the IgE-binding epitopes. Three regions, DDSPDLPKLKPDPNTLC (aa107-123), PHACYTSVFDKLKHLVDEP (aa364-382), and LSLILNRLC (aa451-459), were found to induce T cell proliferation in more than half of the patients tested. Of interest was that these three regions were also recognized by B cells. Information concerning human B and T cells epitopes can contribute greatly to the elucidation of the etiology of beef allergy.
PMID: 12054661 [PubMed - indexed for MEDLINE]
******************
Ob es nicht bezeichnend ist, dass es keine Publikation dazu in Deutscher Sprache gibt???????
fragt sich
Uli
mein englisch ist leider gerade so urlaubsreif 
